Coding

Part:BBa_K2779003

Designed by: Ethan Agena, Rochelin Dalangin, Anna Kim, Irene Shkolnikov   Group: iGEM18_UAlberta   (2018-08-16)


HemC


This enzyme is part of Team UAlberta's engineered biosynthetic pathway to produce protoporphyrin IX (PPIX). Expression of this gene in pBAD for protein purification provided functional hydroxymethylbilane that was used for the in vitro production of PPIX from 5-aminolevulinic acid.

This gene encodes for porphobilinogen deaminase from Escherichia coli strain K12; EC 2.5.1.61 which is a transferase. It catalyzes the 4-porphobilinogen hydroxymethylbilane as shown by the reaction scheme below:

T--UAlberta--HemC Basic Part.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None